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human fcαri (cd89) transgenic scid mice  (Janvier Labs)
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Janvier Labs human fcαri (cd89) transgenic scid mice
Mouse-Ly-6G efficiently depleted neutrophils in C57Bl6/J, Balb/c, NXG, and <t>SCID</t> mice. ( a ) Experimental set-up; i.p. injections with the depletion antibodies were performed three times a week for four weeks. Blood was drawn via cheek puncture once a week before injection with the antibodies; ( b , c ) Longitudinal analysis of the number of CD45 + Siglec-F − CD115 − SSC high Gr-1 + CD11b + neutrophils in the peripheral blood ( n = five mice per group) per 5000 latex beads, showing; ( b ) Significant, almost complete, neutrophil depletion in all mouse strains tested (C57Bl6/J, Balb/c, NXG, and SCID) upon treatment with 100 μg mouse-Ly-6G antibody, and ( c ) Significantly better neutrophil depletion in 100 μg mouse-Ly-6G treated C57Bl6/J mice than when treated with 100 μg rat-Ly-6G antibody. Data is presented as mean with SEM. Statistics: one-way ANOVA with Bonferroni correction. Repeated measures one-way ANOVA with Bonferroni correction was used to compare groups in c, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.
Human Fcαri (Cd89) Transgenic Scid Mice, supplied by Janvier Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mouse-Ly-6G efficiently depleted neutrophils in C57Bl6/J, Balb/c, NXG, and SCID mice. ( a ) Experimental set-up; i.p. injections with the depletion antibodies were performed three times a week for four weeks. Blood was drawn via cheek puncture once a week before injection with the antibodies; ( b , c ) Longitudinal analysis of the number of CD45 + Siglec-F − CD115 − SSC high Gr-1 + CD11b + neutrophils in the peripheral blood ( n = five mice per group) per 5000 latex beads, showing; ( b ) Significant, almost complete, neutrophil depletion in all mouse strains tested (C57Bl6/J, Balb/c, NXG, and SCID) upon treatment with 100 μg mouse-Ly-6G antibody, and ( c ) Significantly better neutrophil depletion in 100 μg mouse-Ly-6G treated C57Bl6/J mice than when treated with 100 μg rat-Ly-6G antibody. Data is presented as mean with SEM. Statistics: one-way ANOVA with Bonferroni correction. Repeated measures one-way ANOVA with Bonferroni correction was used to compare groups in c, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.

Journal: Cells

Article Title: Effective, Long-Term, Neutrophil Depletion Using a Murinized Anti-Ly-6G 1A8 Antibody

doi: 10.3390/cells11213406

Figure Lengend Snippet: Mouse-Ly-6G efficiently depleted neutrophils in C57Bl6/J, Balb/c, NXG, and SCID mice. ( a ) Experimental set-up; i.p. injections with the depletion antibodies were performed three times a week for four weeks. Blood was drawn via cheek puncture once a week before injection with the antibodies; ( b , c ) Longitudinal analysis of the number of CD45 + Siglec-F − CD115 − SSC high Gr-1 + CD11b + neutrophils in the peripheral blood ( n = five mice per group) per 5000 latex beads, showing; ( b ) Significant, almost complete, neutrophil depletion in all mouse strains tested (C57Bl6/J, Balb/c, NXG, and SCID) upon treatment with 100 μg mouse-Ly-6G antibody, and ( c ) Significantly better neutrophil depletion in 100 μg mouse-Ly-6G treated C57Bl6/J mice than when treated with 100 μg rat-Ly-6G antibody. Data is presented as mean with SEM. Statistics: one-way ANOVA with Bonferroni correction. Repeated measures one-way ANOVA with Bonferroni correction was used to compare groups in c, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.

Article Snippet: Experiments were conducted using C57Bl/6J (C57Bl/6JRj), Balb/c (Balb/cByJRj), NXG (NOD.Prkdc scid Il2rg tm1 /Rj), SCID (NOD.CB17-Prkdc scid/scid /Rj), and human FcαRI (CD89) transgenic SCID mice (all housed and bred at Janvier Labs, Paris, France) [ ], or C57Bl/6J FcRγ −/− (C57Bl/6JRj FcRγ-chain knockout) mice (housed and bred at the University of Utrecht) [ ].

Techniques: Injection

Mouse-Ly-6G efficiently depletes neutrophils in the blood and tumor of IMR32 tumor-bearing SCID mice ( a ) Experimental set-up; 2.5 × 10 6 IMR32 cells were s.c. injected in SCID mice in a 1:2 mix with Vitrogel Hydrogel Matrix. Tumors were established for 28 days, after which mice were randomized over the different treatment groups, and treatment was started. I.p. injections with PBS, IgA ch14.18, and mouse-Ly-6G were performed three times a week for six weeks. The SIRPα-D1 fusion protein was injected i.p. every nine days. Blood was drawn via cheek puncture once a week before injection with the antibodies; ( b ) Longitudinal analysis of the percentage of SSC high Ly-6C low Ly-6G + CD11b + neutrophils in the peripheral blood ( n = 2–5 mice per group) of the CD45+ leukocyte population, showing complete neutrophil depletion upon treatment with 100 μg mouse-Ly-6G antibody. Of note; blood at day 70 was collected from the orbit and processed in a similar fashion as the tumor material, meaning cells were fixed at a later time point, possibly resulting in some neutrophil cell death; ( c ) Intratumoral analysis of the percentage of SSC high Ly-6C low Ly-6G + CD11b + neutrophils of the live (TO-PRO-3 negative) cells, CD45+ leukocytes, and CD11b+ myeloid populations. Data showed increased tumor infiltration when the mice were treated with IgA ch14.18/SIRPα-D1 fusion, which was completely ablated when 100 μg mouse Ly-6G was added. Data is presented as mean with SEM. Statistics: one-way ANOVA with Bonferroni correction. Repeated measures one-way ANOVA with Bonferroni correction was used to compare groups in b, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.

Journal: Cells

Article Title: Effective, Long-Term, Neutrophil Depletion Using a Murinized Anti-Ly-6G 1A8 Antibody

doi: 10.3390/cells11213406

Figure Lengend Snippet: Mouse-Ly-6G efficiently depletes neutrophils in the blood and tumor of IMR32 tumor-bearing SCID mice ( a ) Experimental set-up; 2.5 × 10 6 IMR32 cells were s.c. injected in SCID mice in a 1:2 mix with Vitrogel Hydrogel Matrix. Tumors were established for 28 days, after which mice were randomized over the different treatment groups, and treatment was started. I.p. injections with PBS, IgA ch14.18, and mouse-Ly-6G were performed three times a week for six weeks. The SIRPα-D1 fusion protein was injected i.p. every nine days. Blood was drawn via cheek puncture once a week before injection with the antibodies; ( b ) Longitudinal analysis of the percentage of SSC high Ly-6C low Ly-6G + CD11b + neutrophils in the peripheral blood ( n = 2–5 mice per group) of the CD45+ leukocyte population, showing complete neutrophil depletion upon treatment with 100 μg mouse-Ly-6G antibody. Of note; blood at day 70 was collected from the orbit and processed in a similar fashion as the tumor material, meaning cells were fixed at a later time point, possibly resulting in some neutrophil cell death; ( c ) Intratumoral analysis of the percentage of SSC high Ly-6C low Ly-6G + CD11b + neutrophils of the live (TO-PRO-3 negative) cells, CD45+ leukocytes, and CD11b+ myeloid populations. Data showed increased tumor infiltration when the mice were treated with IgA ch14.18/SIRPα-D1 fusion, which was completely ablated when 100 μg mouse Ly-6G was added. Data is presented as mean with SEM. Statistics: one-way ANOVA with Bonferroni correction. Repeated measures one-way ANOVA with Bonferroni correction was used to compare groups in b, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.

Article Snippet: Experiments were conducted using C57Bl/6J (C57Bl/6JRj), Balb/c (Balb/cByJRj), NXG (NOD.Prkdc scid Il2rg tm1 /Rj), SCID (NOD.CB17-Prkdc scid/scid /Rj), and human FcαRI (CD89) transgenic SCID mice (all housed and bred at Janvier Labs, Paris, France) [ ], or C57Bl/6J FcRγ −/− (C57Bl/6JRj FcRγ-chain knockout) mice (housed and bred at the University of Utrecht) [ ].

Techniques: Injection