Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Alignment of CD89 derived amino acid sequences obtained by cloning and sequencing rhesus macaque (GenBank accession number AY386684) and cynomolgus macaque (GenBank accession number AY386690) cDNA from whole blood and comparison with the published human sequence (GenBank NM_002000). Amino acid differences are underlined. The first amino acid of the preprotein is numbered as residue 1. The mature peptide starts at residue 22. Arrows indicate distinct domains. The first two amino acids for EC1 are encoded at the end of the S2 exon. The signal peptide is encoded by both S1 and S2 sequences. Potential N-glycosylation sites, cysteines involved in disulfide bonds and arginine 209 critical for association with the FcRγ chain are bolded. Hu: Homo sapiens; Mamu: Macaca mulatta; Mafa: Macaca fascicularis.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques: Derivative Assay, Clone Assay, Sequencing

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Expression of recombinant macaque CD89 on HeLa cells. Cells were transfected with a plasmid containing full-length (a) rhesus macaque CD89 cDNA or (b) cynomolgus macaque CD89 cDNA and stained with anti-human CD89: thick line (rhesus MFI = 120·70, SD = 343·04; cynomolgus 30·35, SD = 345·04) or with isotype control mouse IgG: thin line (rhesus MFI = 8·65, SD = 7·40; cynomolgus MFI = 9·27, SD = 34·47). Untransfected HeLa cells stained with anti-human CD89: dotted line (MFI = 5·26, SD = 28·69). MFI = Mean fluorescence intensity, SD = standard deviation. Stable transfectants were used for detection of rhesus macaque CD89 and transient transfectants were used for detection of cynomolgus macaque CD89. 5000 events were counted per sample.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques: Expressing, Recombinant, Transfection, Plasmid Preparation, Staining, Fluorescence, Standard Deviation

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Alignment of the five rhesus macaque CD89 splice variants (GenBank accession number AY386684-AY386689) with full-length CD89 from the same species. Arrows indicate distinct domains. Mamu: Macaca mulatta.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques:

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Alignment of the three cynomolgus macaque CD89 splice variants (GenBank accession number AY386690-AY386693) with full-length CD89 from the same species. Arrows indicate distinct domains. Mafa: Macaca fascicularis.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques:

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Schematic representation of the complete CD89 transcript and corresponding splice variants identified in rhesus and cynomolgus macaques. Mamu: Macaca mulatta; Mafa: Macaca fascicularis.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques:

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Two-colour dot-plots of whole blood leucocytes from a representative rhesus (a–d) and cynomolgus macaque (e–h). Forward scatter (FSC) versus side scatter (SSC) (a and e); CD89 versus CD20 (b and f); CD3 (c and g); and CD16 (d and h).

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques:

Journal:

Article Title: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor)

doi: 10.1111/j.1365-2567.2004.01949.x

Figure Lengend Snippet: Two-colour dot-plots of whole-blood leucocytes from a representative rhesus (a–d) and cynomolgus macaque (e–h). (a and e) CD14 versus side scatter. CD14 versus CD89: (b and f) total leucocytes; (c and g) granulocytes; (d and h) monocytes. Gates used for granulocyte (R1) and monocyte (R2) populations of rhesus (a) and cynomolgus macaque (e) are shown.

Article Snippet: 1 × 10 6 cells were stained at 4° with either 20 µl of phycoerythrin-conjugated mouse anti-human CD89 (clone A59) or Simultest TM Control γ 1 /γ 2a (both from BD PharMingen, San Diego, CA) for 15 min, followed by three washes with PBS.

Techniques:

Journal: bioRxiv

Article Title: IgA Potentiates NETosis in Response to Viral Infection

doi: 10.1101/2021.01.04.424830

Figure Lengend Snippet: (A) Primary human neutrophils were stimulated for 3 hours with antibody:IAV ICs generated from matched salivary IgA (sIgA) and serum IgA of healthy IAV-exposed donors (n=4). (B) Primary human neutrophils were incubated with an anti-CD89 (FcαRI) antibody prior to stimulation with IgG:IAV or IgA:IAV ICs (n=3) (C) Primary human neutrophils were stimulated with polyclonal IgA, polystyrene beads coated with Protein L, or polystyrene beads coated with protein L and IgA. For all experiments, NETosis was assessed by immunofluorescence microscopy analysis of cells co-stained for DNA (DAPI) and neutrophil elastase. NETosis in stimulated conditions was normalized to untreated cells (n=6). (D) Fluorescent polystyrene beads were coated with protein L, followed by either polyclonal IgG or IgA. Human neutrophils were isolated and incubated with the beads at a 500 beads/cell ratio. After washing, phagocytosis of beads was measured using a SpectraMax i3 plate reader (Molecular Devices) (n=3). (E) Purified Cal/09 was immobilized on glass coverslips prior to the addition of IgG or IgA. Primary human neutrophils were added to wells for 3 hours before being fixed and stained for quantification (n=3). Mean and SEM of independent experiments are shown. Statistical significance was evaluated by one-way ANOVA and Tukey post-hoc test. *, P < 0.05; **, P < 0.01.

Article Snippet: To block FcαRI, 20 μg/mL of mouse anti-human CD89 antibody (AbD Serotec) was added to neutrophils for 20 minutes at 4°C.

Techniques: Generated, Incubation, Immunofluorescence, Microscopy, Staining, Isolation, Purification

Journal: BMC Immunology

Article Title: C-reactive protein (CRP) but not the related pentraxins serum amyloid P and PTX3 inhibits the proliferation and induces apoptosis of the leukemia cell line Mono Mac 6

doi: 10.1186/s12865-017-0230-z

Figure Lengend Snippet: CRP receptor expression on 5 leukemia cell lines. CD16, CD32, CD64, CD89, and CD209 expression on the indicated cell lines was examined by flow cytometry. Top row: Blue trace is CD64, red is CD89. Middle row: Blue is CD16, red is CD209. Bottom row: Blue is CD32-Fun2, Red is CD32-IV3. Black traces are control IgGs. Plots are representative of 3 independent experiments

Article Snippet: Mouse monoclonal antibodies to CD16 (GRM1 and 3G8, mouse IgG1 BioLegend), CD32 (Fun2; AT10 mouse IgG1, BioRad; and 6C4, mouse IgG1, eBioscience, Rockford, IL), CD64 (clone 10.1), and CD89 (clone A59), mouse IgG1, IgG2a, and IgG2b (all from BioLegend), and human Fc blocker (BD Biosciences) or human Fc receptor binding inhibitor (eBioscience) diluted to a final concentration of 10 μg/ml were added to Mono Mac 6 cells in the presence or absence of CRP.

Techniques: Expressing, Flow Cytometry, Control

Journal: BMC Immunology

Article Title: C-reactive protein (CRP) but not the related pentraxins serum amyloid P and PTX3 inhibits the proliferation and induces apoptosis of the leukemia cell line Mono Mac 6

doi: 10.1186/s12865-017-0230-z

Figure Lengend Snippet: Anti-CD64 antibodies attenuate the CRP effect on Mono Mac 6 cells. Anti-CD32, anti-CD64, anti-CD89 antibodies, mouse IgG isotypes, or 2 Fc blockers were added to Mono Mac 6 cells for 2 h before 75 μg/ml CRP was added to the cells. Cell densities at 48 h were normalized to the density with CRP alone. Values are mean ± SEM, n = 3 independent experiments. ** indicates p < 0.01 (t-test)

Article Snippet: Mouse monoclonal antibodies to CD16 (GRM1 and 3G8, mouse IgG1 BioLegend), CD32 (Fun2; AT10 mouse IgG1, BioRad; and 6C4, mouse IgG1, eBioscience, Rockford, IL), CD64 (clone 10.1), and CD89 (clone A59), mouse IgG1, IgG2a, and IgG2b (all from BioLegend), and human Fc blocker (BD Biosciences) or human Fc receptor binding inhibitor (eBioscience) diluted to a final concentration of 10 μg/ml were added to Mono Mac 6 cells in the presence or absence of CRP.

Techniques:

Journal: BMC Immunology

Article Title: C-reactive protein (CRP) but not the related pentraxins serum amyloid P and PTX3 inhibits the proliferation and induces apoptosis of the leukemia cell line Mono Mac 6

doi: 10.1186/s12865-017-0230-z

Figure Lengend Snippet: CRP regulates receptor expression on Mono Mac 6 cells. a Flow cytometry analysis of CD64 and CD89 expression on Mono Mac 6 cells in the presence or absence of CRP at 48 h. b Quantification of mouse IgG isotype, CD32, CD64, and CD89 antibody binding to Mono Mac 6 cells after incubation with or without CRP for 48 h. Values are mean ± SEM, n = 4 independent experiments. ** indicates p < 0.01 (t-test)

Article Snippet: Mouse monoclonal antibodies to CD16 (GRM1 and 3G8, mouse IgG1 BioLegend), CD32 (Fun2; AT10 mouse IgG1, BioRad; and 6C4, mouse IgG1, eBioscience, Rockford, IL), CD64 (clone 10.1), and CD89 (clone A59), mouse IgG1, IgG2a, and IgG2b (all from BioLegend), and human Fc blocker (BD Biosciences) or human Fc receptor binding inhibitor (eBioscience) diluted to a final concentration of 10 μg/ml were added to Mono Mac 6 cells in the presence or absence of CRP.

Techniques: Expressing, Flow Cytometry, Binding Assay, Incubation

Journal: BMC Immunology

Article Title: C-reactive protein (CRP) but not the related pentraxins serum amyloid P and PTX3 inhibits the proliferation and induces apoptosis of the leukemia cell line Mono Mac 6

doi: 10.1186/s12865-017-0230-z

Figure Lengend Snippet: CRP receptor expression on 5 leukemia cell lines. CD16, CD32, CD64, CD89, and CD209 expression on the indicated cell lines was examined by flow cytometry. Top row: Blue trace is CD64, red is CD89. Middle row: Blue is CD16, red is CD209. Bottom row: Blue is CD32-Fun2, Red is CD32-IV3. Black traces are control IgGs. Plots are representative of 3 independent experiments

Article Snippet: CRP and mouse monoclonal antibodies to CD32 (clone Fun2), CD64 (clone 10.1), or CD89 (clone A59) were also added to Mono Mac 6 cells in the presence or absence of 10 μg/ml donkey anti-mouse antibodies (Jackson ImmunoResearch) to cross-link the antibodies.

Techniques: Expressing, Flow Cytometry, Control

Journal: BMC Immunology

Article Title: C-reactive protein (CRP) but not the related pentraxins serum amyloid P and PTX3 inhibits the proliferation and induces apoptosis of the leukemia cell line Mono Mac 6

doi: 10.1186/s12865-017-0230-z

Figure Lengend Snippet: Anti-CD64 antibodies attenuate the CRP effect on Mono Mac 6 cells. Anti-CD32, anti-CD64, anti-CD89 antibodies, mouse IgG isotypes, or 2 Fc blockers were added to Mono Mac 6 cells for 2 h before 75 μg/ml CRP was added to the cells. Cell densities at 48 h were normalized to the density with CRP alone. Values are mean ± SEM, n = 3 independent experiments. ** indicates p < 0.01 (t-test)

Article Snippet: CRP and mouse monoclonal antibodies to CD32 (clone Fun2), CD64 (clone 10.1), or CD89 (clone A59) were also added to Mono Mac 6 cells in the presence or absence of 10 μg/ml donkey anti-mouse antibodies (Jackson ImmunoResearch) to cross-link the antibodies.

Techniques:

Journal: BMC Immunology

Article Title: C-reactive protein (CRP) but not the related pentraxins serum amyloid P and PTX3 inhibits the proliferation and induces apoptosis of the leukemia cell line Mono Mac 6

doi: 10.1186/s12865-017-0230-z

Figure Lengend Snippet: CRP regulates receptor expression on Mono Mac 6 cells. a Flow cytometry analysis of CD64 and CD89 expression on Mono Mac 6 cells in the presence or absence of CRP at 48 h. b Quantification of mouse IgG isotype, CD32, CD64, and CD89 antibody binding to Mono Mac 6 cells after incubation with or without CRP for 48 h. Values are mean ± SEM, n = 4 independent experiments. ** indicates p < 0.01 (t-test)

Article Snippet: CRP and mouse monoclonal antibodies to CD32 (clone Fun2), CD64 (clone 10.1), or CD89 (clone A59) were also added to Mono Mac 6 cells in the presence or absence of 10 μg/ml donkey anti-mouse antibodies (Jackson ImmunoResearch) to cross-link the antibodies.

Techniques: Expressing, Flow Cytometry, Binding Assay, Incubation